Malin Edvardsson Oct 8, ’19 > 4 min

8 publications you should read if you are working with formation of supported lipid bilayers

Bilayer formation from vesicles has long been known to occur on silica/glass surfaces, but the mechanism was for a long time unknown. By QCM-D measurements, a major step was taken in the understanding of the bilayer formation process and regarding the possibilities to control the process on different surfaces and for different lipids. Here we list eight key publications that you should read if you want to get on top of the key facts and fundamentals of the bilayer formation process.

The supported lipid bilayer as a cell membrane mimic

A common underlying motivation to prepare and study supported lipid bilayers (SLB) is to prepare a model system mimicking cell membranes. The SLB formation is the first step in making more cell-membrane like model membranes, where additional steps are incorporation of functional membrane-bound molecules such as peptides, proteins, glycolipids etc, onto and/or into the membrane. One branch of SLB research today is successively moving towards much more complex systems.

Key parameters that influence the bilayer formation

Both the lipid bilayer formation dynamics and the quality of the formed lipid layer are affected by factors such as lipid composition, substrate material, temperature and solvent properties. There are several research studies on bilayer formation, where QCM-D was used as a central analysis method, which have thoroughly explored the key influential parameters and mapped out the fundamentals of how the lipid bilayer formation process is affected by the formation conditions. Below we list eight of these publications, which we think are particularly relevant, and which we think you should read if you are in this field of research.

 List of publications

  1. Surface specific kinetics of lipid vesicle adsorption measured with a quartz crystal microbalance
    Keller, CA and Kasemo, B, Biophysical Journal, 75, 3 (1998), pp 1397 – 1402

  2. Intact vesicle adsorption and supported biomembrane formation from vesicles in solution: influence of surface chemistry, vesicle size, temperature and osmotic pressure
    Reimhult, E. et al., Langmuir, 19, 5 (2003), pp 1681 – 1691

  3. Pathways of lipid vesicle deposition on solid surfaces: A combined QCM-D and AFM study
    Richter, R., et al., Biophysical Journal, 85, 5 (2003), pp 3035-3047

  4. Simultaneous surface plasmon resonance and quartz crystal microbalance with dissipation monitoring measurements of biomolecular adsorption events involving structural transformations and variations in coupled water
    Reimhult, E., et al., Analytical Chemistry, 76, 24 (2004), pp 7211-7220

  5. Formation of Supported bilayers on silica substrates
    Anderson, T. H., et al., Langmuir, 25, 12 (2009), pp 6997-7005

  6. Influence of mono- and divalent ions on the formation of supported phospholipid bilayers via vesicle adsorption
    Seantier, B. and Kasemo, B., Langmuir, 19, 25 (2009), pp 5767-5772

  7. Quartz crystal microbalance with dissipation monitoring of supported lipid bilayers on various substrates
    Cho, NJ., et al., Nature protocols, 5, 6 (2010), pp 1096-1106

  8. Influence of Divalent Cations on Deformation and Rupture of Adsorbed Lipid Vesicles
    Dacic, M., et al.,
    Langmuir, 32, 25 (2016), pp 6486-6495.    

Download the list as pdf below

Overview  8 Key QCM-D publications on the fundamentals of SLB formation  Download

 

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