Bilayer formation from vesicles has long been known to occur on silica/glass surfaces, but the mechanism was for a long time unknown. By QCM-D measurements, a major step was taken in the understanding of the bilayer formation process and regarding the possibilities to control the process on different surfaces and for different lipids. Here we list eight key publications that you should read if you want to get on top of the key facts and fundamentals of the bilayer formation process.
The supported lipid bilayer as a cell membrane mimic
A common underlying motivation to prepare and study supported lipid bilayers (SLB) is to prepare a model system mimicking cell membranes. The SLB formation is the first step in making more cell-membrane like model membranes, where additional steps are incorporation of functional membrane-bound molecules such as peptides, proteins, glycolipids etc, onto and/or into the membrane. One branch of SLB research today is successively moving towards much more complex systems.
Key parameters that influence the bilayer formation
Both the lipid bilayer formation dynamics and the quality of the formed lipid layer are affected by factors such as lipid composition, substrate material, temperature and solvent properties. There are several research studies on bilayer formation, where QCM-D was used as a central analysis method, which have thoroughly explored the key influential parameters and mapped out the fundamentals of how the lipid bilayer formation process is affected by the formation conditions. Below we list eight of these publications, which we think are particularly relevant, and which we think you should read if you are in this field of research.
Lipid-based systems such as vesicles, monolayers and bilayers are interesting as model membrane platforms and are used in various research areas. To characterize these systems, it is often necessary to use more than one analysis method. So which analysis methods should you use?
Lipid-based systems are used in various fields of research, e.g. in the design and development of biosensor platforms, biomaterial coatings and drug delivery applications. In this overview, we present examples of how these lipid-based systems can be characterized using QSense QCM-D technology.