Quantifying QCM mass, there are two different approaches to choose from depending on whether the layer is rigid of soft - the Sauerbrey equation or viscoelastic modeling. But what if the wrong model is applied, what happens then? How critical are the consequences? Here we describe what happens if the wrong quantification approach is used.
Using Sauerbrey when viscoelastic modeling should be applied
Of the two situations, using the Sauerbrey approach on a viscoelastic layer is the most severe one. Since the Sauerbrey equation is not valid for viscoelastic layers, the calculated mass will be incorrect. Not only will the mass be underestimated, but it will also vary depending on which harmonic that was used for the calculation. An example of this situation is illustrated in Fig. 1, where the results from Sauerbrey quantification and viscoelastic modeling of a soft layer are compared. As shown in the figure, the calculated Sauerbrey mass depends on which of the harmonics that was used as input. A key signature of a soft layer is that the harmonics are spreading, and the Sauerbrey mass will, therefore, depend on which harmonic that was used for the calculation.
As can also be observed in Fig. 1, none of the calculated Sauerbrey masses are close to the viscoelastic modeled one, they are all underestimated. This means that for soft layers, the Sauerbrey approach cannot even be used to get an estimation of the mass.
Consequences of using the Sauerbrey equation on a soft layer
The calculated mass depends on which harmonic that was used as input
The calculated mass is underestimated for all harmonics
Figure 1. Mass calculated for a viscoelastic layer, comparing the results from Sauerbrey calculations using harmonics n = 1 - 13 and viscoelastic modeling.
Using viscoelastic modeling when the Sauerbrey approach should be applied
Using viscoelastic modeling on a rigid layer is less severe than the other way around. In this situation, the biggest problem will be for the modeling software to find a good fit. To model the QCM-D data, unique input from at least two harmonics is needed, and since the overtones are overlapping for rigid films, they will not provide enough unique information to use as input in the model. The consequence can sometimes, therefore, be that the software will not succeed in finding a solution. If it does, the modelled viscosity and shear values may be random.
Consequences of using viscoelastic modeling on a rigid layer
It can be difficult to model the data and find a good fit
Modeled viscosity and shear values may be random
If the layer to be analyzed fulfills the modeling requirements, there are two approaches that can be used depending on whether the layer is rigid or soft. Applying the rigid layer approach on a soft layer will give the wrong result and is more severe than the other way around where the main consequence is that it can be difficult to find a good fit.
Download the guide to get a summary on how to assess which analysis method to use.
Surveying the market of QCM:s, one will come across a vast range of different QCM versions, and they all seem to provide similar information. What are the differences, and does it matter which one I use?
Lipid-based systems are used in various fields of research, e.g. in the design and development of biosensor platforms, biomaterial coatings and drug delivery applications. In this overview, we present examples of how these lipid-based systems can be characterized using QSense QCM-D technology.